Cryopreservation techniques in genipap and mangaba: challengers and perspectives


  • Anna Beatriz Nogueira de Araújo
  • Annie Carolina Araújo de Oliveira
  • Genilza Almeida da Graça
  • Ana Veruska Cruz da Silva
  • Ana da Silva Lédo



Genipa americana L, Hancornia speciosa Gomes, cryopreservation, encapsulation, ex situ conservation


The genipap (Genipa americana L.) is a native species of Brazil, found in various regions of the country, from the North to the South, including Sergipe. On the other hand, the mangabeira (Hancornia speciosa Gomes), another Brazilian tropical plant, is recognized for its fruits rich in iron and vitamin C, widely exploited by the pulp, juice, and ice cream industry. However, it faces threats due to urban development in the coastal region. To preserve these species, techniques such as cryopreservation are fundamental. This approach, which maintains plant tissues at ultra-low temperatures, allows the conservation of genetic material indefinitely, contributing to the maintenance of biological diversity. Encapsulation technology, which involves enclosing plant material in a specific matrix, is also promising in this context, offering a long-term preservation method for plant explants and embryos. These methods represent significant advances in the field of plant species conservation, providing effective strategies to ensure the survival of these plants in the face of environmental challenges and human development. The objective of this review is to address some studies involving cryopreservation techniques for genipap and mangaba species. Through cryopreservation techniques, we can achieve ex situ conservation for the germplasm bank as well as for plant regeneration and reforestation of areas. It is evident that there is a need to increase studies on the conservation of both species.


ARA, H.; JAISWAL, U.; JAISWAL, V. S. Synthetic seeds: perspectives and limitations. Current Science, v. 78, p. 1438-1444, 2000.

ARMITAGE, W. J., RICH, S. J. Vitrification of organized tissues. Cryobiology, v. 27, p. 483-491, 1990.

ARNAO, M.T.G.; PANTA, A.; ROCA, W.M.; ESCOBAR, R.H.; ENGELMANN, F. Development and large-scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops. Plant Cell Tissue Organ Culture, v. 92, p.1-13, 2008.

AHMED, M. D. R.; ANIS, M.; AL-ETTA, H. A. Encapsulation technology for short-term storage and germplasm exchange of Vitex trifolia L. Rendiconti Lincei, v. 26, n. 2, p. 133–139, 2015.

BENELLI, C.; CARLO, A.; ENGELMANN, F. Recent advances in the cryopreservation of shoot-derived germplasm of economically important fruit trees of Actinidia, Diospyros, Malus, Olea, Prunus, Pyrus and Vitis. Biotechnology Advances, v. 31, p. 171-185, 2013.

CARVALHO, J. E. U. DE; NASCIMENT0, W. M. O. DO SENSITIVITY OF JENIPAP SEEDS (Genipa americana L.) TO DEHYDRATION AND FREEZING. Revista Brasileira de Fruticultura. Jaboticabal - SP. v. 22. 11. I. p. 53-56. abril 2000.

CORRÊA, P. M. Dictionary of useful plants of Brazil and cultivated exotics. Rio de Janeiro: IBDF, 1984.

DELPRETE, P. G.; SMITH, L. B.; KLEIN, R. M. Illustrated Flora of Santa Catarina: Rubiaceae. Itajaí: Herbário Barbosa Rodrigues, 2005.

DJERASSI, C.; GRAY, J. D.; KINCL, F. A. Naturally occurring oxygen heterocyclies: isolation and characterization of genipin. The Journal of Organic Chemistry, v. 25, p. 2174-2177, 1960.

ENGELMANN, F. Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cell & Developmental Biology-Plant, Berlin, v. 47, n. 4, p. 5-16, 2011.

FEBRE, J.; DEREUDDRE, J. Encapsulation-dehydration: A new approach to cryopreservation of solanum shoot-tips. Cryoletters, v. 11, p. 413–426, 1990.

FIGUEIREDO, J. R. M.; PAIVA, P. D. O.; PAIVA, R.; DA SILVA; D. P. C.; MESQUITA, R.; DE FARIAS, C. V. N.; REIS, M. V. Conservation of Genipa americana: encapsulation and slow growth techniques. Revista eletrônica da Universidade Vale do Rio Verde, v. 16, n. 1, 2018.

FONSECA, F. K. P.; LEMOS, E. E.; OLIVEIRA, G. L.; ALENCAR, L. M. C. Effect of hormonal balance on organogenesis and multiplication of shoots of Hancomia speciosa Gomes in vitro. In: Brazilian Symposium on the Cultivation of Mangaba, 2003, Aracaju. Annals. Aracaju: Embrapa Coastal Tablelands, 2003. CD-ROM. Expanded Abstracts Section.

GOMES, M. Genipa Flora and Fungi of Brazil. Rio de Janeiro Botanical Garden, 2015. Available at: Accessed on: Feb 13, 2023.

GUARNACCIA, R.; MADYASTHA, K. M.; TEGTMEYER, E.; COSCIA, C. J. Geniposidic acid, an iridoid glucoside from Genipa americana. Tetrahedron Lett, v. 50, p. 5125-5127, 1972.

GUERRA, M. P.; DAL VESCO, L. L.; DU CROQUET, P. H. J.; NODARI, R.; REIS, M. S. Somatic embryogenesis in goiabeira serrana: genotype response, auxinic hock and synthetic seeds. Revista Brasileira de Fisiologia Vegetal, v. 13, n. 2, p. 117-128, 2001.

JENSEN, S. R. Iridoids in Rothmannia globosa. Phytochemistry, v. 22, p. 1761-1765, 1983.

KITTO, S. K.; JANICK, J. Polyox as an artificial seed coat for asexual embryos. Horticultural Science, v. 17, p. 488, 1982

KOHOMA, S.; MALUF, A. M.; BILIA, D. A. C. Drying and storage of seeds of Eugenia brasiliensis Lam. (Grumixameira). Revista Brasileira de Sementes, Pelotas, v. 28, n. 1, p. 72-78, abr. 2006.

LAMEIRA, O. A.; LEMOS, O. F.; MENEZES, I. C. de; PINTO, J. E. B. P. Tissue culture (manual). Belém: Embrapa Amazônia Oriental, 2000.

LIMA, F. Cryopreservation of plants is a novelty at EMBRAPA. Technology allows for the conservation of meristems, embryos, seeds, or other plant tissues indefinitely. Embrapa Temperate Climate, 2014.

MONDO, V. H. V.; CICERO, S. M. Aspects of synthetic seed technology. Informativo Abrates, v. 18, n. 1, 2, 3, p. 23-29, 2008.

NASCIMENTO, C. M. DO; OLIVEIRA, L. A. R. DE; SILVA, A. V. C. DA; CASTRO, E. M. DE; LÉDO, A. DA S. Long term conservation of embryonic axes of genipap accessions. Scientia Plena, 16(2), 2020.

NISHIZAWA, S.; SAKAI, A.; AMANO, Y.; MATSUZAWA, T. Cryopreservation of asparagus (Asparagus officinalis L.) embryogenic suspension cells and subsequent plant regeneration by vitrification. Plant Science, v. 91, n. 1, p. 67–73, 1993.

NOGUEIRA, G. F. Cryopreservation and in vitro synthetic seed production of mangaba. 2010. 72p.:il. Dissertation (Master's) – Federal University of Lavras, UFLA, UFLA, Minas Gerais, 2010.

PANIS, B.; LAMBARDI, M. Status of cryopreservation technologies in plants: crops and forest trees. In: Eletronic forum on biotechnology in food and agriculture, 13., 2005, Rome. Proceedings. Rome: FAO, 2005.

PEREIRA, J. E.; GUEDES, R. D. S.; COSTA, F. H. D. S.; SCHMITZ, G. C. B. Composition of the encapsulation matrix in the formation and conversion of synthetic seeds of long pepper. Horticultura Brasileira, v.26, p. 93-96, 2008.

PINTOS, B., BUENO, M. A., CUENCA, B., & MANZANERA, J. A. Synthetic seed production from encapsulated somatic embryos of cork oak (Quercus suber L.) and automated growth monitoring. Plant Cell, Tissue and Organ Culture, v. 95, p. 217-225, 2008.

RAI, M. K.; ASTHANA, P.; SINGH, S. K.; JAISWAL, V. S.; JAISWAL, U. The encapsulation technology in fruit plants—a review. Biotechnology Advances, v. 27, p. 6, p. 671-679, 2009.

REVILLA, J. Plants of the Amazon. Manaus: Business and Technology Development Program, 2001.

RUZZA, D. A. C.; ROSSI, A. A. B.; FERNANDES, J. M.; DE PEDRI, E. C. M.; TIAGO, A. V., BISPO, R. B.; MARTINS, K. C. Ethnobotany of jenipapo (Genipa americana L., Rubiaceae) among farmers in the municipality of Carlinda, Mato Grosso, Brazil. Brazilian Journal of Development, v. 6, n. 8, p. 61161-61184, 2020.

SÁ, F. P.; SOUZA, V. F. D.; SILVA, V. C.; LÉDO, S. A. Encapsulation, cryoprotection, and dehydration in the regenerative capacity of caulinar apices of Genipa americana. Rural Science, Santa Maria, v. 45, n. 11, p. 1939-1945, 2015.

SARTOR, F. R.; MORAES, A. M. de; ALMEIDA, F. A. C. Techniques for cryopreservation of mangaba buds. Agrotechnology Journal, Anápolis, v. 3, n. 1, p. 31-39, 2012.

SANTANA, F. V.; OLIVEIRA, L. A. R. de; SANTOS, P. A. A.; SILVA, A. V. C. da; LEDO, A. da S. Effects of droplet vitrification on the regeneration of apical tips of mangaba accessions. Investigation, Society and Development, v. 11, n. 17, e38111738734, 2022.

SANTOS, I. R. I.; SALOMÃO, A. N. Viability assessment of Genipa americana L. (Rubiaceae) embryonic axes after cryopreservation using in vitro culture. International Journal of Agronomy, London, v. 2016, p. 1-6, 2016.

SANTOS, P. A. A.; PAIVA, R.; SILVA, L. C.; SOUZA, A. C.; SANTANA, M. C.; SILVA, D. P. C. Cryopreservation of the mangaba tree (Hancornia speciosa Gomes): a protocol for long-term storage. Acta Scientiarum. Agronomy, Maringa, v. 37, p. 289-296, 2015.

SANTOS, A. R. F. dos; SILVA-MANN, R.; FERREIRA, R. A. Water restriction in Genipa seeds (Genipa americana L.). Revista Árvore, v. 35, n. 2, Apr. 2011.

SANTOS, IRI; SALOMAO, A. N. Practical guide to cryopreservation: techniques applied to the conservation of plant germplasm. Brochures, Embrapa Genetic Resources and Biotechnology. Documents, 385, 2023.

SAKAI, A.; HIRAI, D.; NIINO, T. Development of PVS-Based vitrification and encapsulation–vitrification protocols. In: REED, B. M. (Ed.). Plant cryopreservation: A Practical Guide. New York, NY: Springer, p. 33–57, 2008.

SOUZA, A. C. D.; COSTA, M. C.; FIGUEIREDO, M. A. D.; PEREIRA, C. C.; COELHO, S. V. B.; VILELA, A. L. O.; ROSA, S. D. V. F. D. Stages of coffee seed cryopreservation: physiological and antioxidant system responses. Ciência Rural, 54, e20220480, 2023.

SILVA JUNIOR, J. F. Mangaba culture. Brazilian Journal of Fruit Growing, v. 26, n. 1, Apr. 2004.

SILVEIRA, A. A. C.; SIBOV, S. T. Encapsulation-dehydration of Eugenia dysenterica caulinar apices. Multi-Science Journal, v. 2, n. 2, p. 39-41, 2019.

TOWILL, L. E. Cryopreservation of plant germplasm. In: TOWILL, L. E.; BAJAJ, Y. P. S. Cryopreservation of plant germplasm II. Berlin: Springer, Biotechnology in Agriculture and Foresty, v. 50, p. 4-21, 2002.

WANG, Q.; LAAMANEN, J.; UOSUKAINEN, M.; VALKONEN, JP. Cryopreservation of in vitro-grown shoot tips of raspberry (Rubus idaeus L.) by encapsulation-vitrification and encapsulation-dehydration. Plant Cell Reports, v. 24, p. 280-288, 2005.



How to Cite

Araújo, A. B. N. de, Oliveira, A. C. A. de, Graça, G. A. da, Silva, A. V. C. da, & Lédo, A. da S. (2024). Cryopreservation techniques in genipap and mangaba: challengers and perspectives. OBSERVATÓRIO DE LA ECONOMÍA LATINOAMERICANA, 22(3), e3675.




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