Modified technique for recovery of nematode infective larvae in different grass genera


  • Gustavo Delmilho
  • Ricardo Lopes Dias da Costa
  • Adibe Luiz Abdalla
  • Waldssimiler Teixeira de Mattos
  • Rafael Sílvio Bonilha Pinheiro



infecting nematodes, ovis aries, parasitology, recovery of larva


Most gastrointestinal nematodes are in the grazing environment. As an option, the study aimed to evaluate an alternative technique for recovery of infecting larvae in various grass genera: Tyfton 85 (Cynodon dactylon) Capim–Vencedor (Megathyrsus maximus) Capim–Gordura (Melinis minutiflora) Capim–Marandu (Brachiaria brizantha), Setaria (Setaria anceps) Capim-Humidicula (Brachiaria humidicula) and Andropogon (Andropogon gayanus). The collection of samples and procedures were developed at the Institute of Animal Science, Nova Odessa, Brazil. The grasses came from isolated paddocks. Three subsamples were used: 1-control sample (AC1); 2-control frozen sample (ACC2); 3-infested sample (AI). AC1, after the process of larvae recoveries, served as control, the rest was frozen. After de-freezing, it was divided into two samples (ACC2 and AI). In ACC2, no larvae were observed. AI was infested with 1600 ± 30 L3 larvae of trichostrongylids, recovered from sheep manure. The larvae recovery procedures were applied to the samples in the three periods. The overall mean of recovery (±SD) and percentage of success was estimated (SISVAR®) in 1544.63 ± 1.31 larvae, 96.54 ± 17% respectively. There were no differences between grass genera, as well as between weeks, with near recovery numbers (p>0.05). The technique had satisfactory results in a short time.


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How to Cite

Delmilho, G., da Costa, R. L. D., Abdalla, A. L., de Mattos, W. T., & Pinheiro, R. S. B. (2024). Modified technique for recovery of nematode infective larvae in different grass genera. OBSERVATÓRIO DE LA ECONOMÍA LATINOAMERICANA, 22(2), e3381.




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