Daphnias spp. as bioindicator organisms of toxicity and environmental characterization of eutrophized aquatic systems


  • Maria Virgínia da Conceição Albuquerque
  • Joelma Dias
  • Fabiane Rabelo da Costa Batista
  • Emmanuelle Marie Albuquerque Oliveira
  • Maria Célia Cavalcante de Paula e Silva
  • Roberta Milena Moura Rodrigues
  • Valderi Duarte Leite
  • Wilton Silva Lopes




Daphnias ssp., ecotoxicity, cyanobacteria


The ecotoxicological study is a tool for determining the harmful effects of chemical agents on the environment, enabling the verification of the ecotoxicological characteristics of chemical substances, mechanisms of action on living organisms, definition of guidelines for controlling the quality of effluents and evaluation of efficiency of water treatment processes. Therefore, this article presents a review on the assessment of ecotoxicity as an environmental characterization tool in eutrophicated aquatic systems using Daphnias spp. as bioindicator organisms. The studies evaluated showed that cyanotoxins cause inhibition of feeding activity, decreased growth, low reproduction rate, poor offspring formation, decreased lifespan and increased tolerance to toxins throughout generations of this microcrustacean. Acute toxicity analyzes showed different toxic effects, as the results depended on the strain tested. Some factors are attributed to the toxicity of cyanobacteria extracts in zooplanktonic organisms, mainly nutritional inadequacy, decreased filtration activity, inhibition of digestive enzymes, bioaccumulation and oxidative stress.


ALBUQUERQUE, Maria Virgínia da Conceição. Assessment of the potential toxic effect of cyanotoxins and their degradation by-products by advanced oxidative processes. 2022. 177. Thesis (Postgraduate Program in Environmental Science and Technology - PPGCTA) - State University of Paraíba, Campina Grande, 2023.


Seasonal variations of morpho-functional phytoplankton groups influence the top-down control of a cladoceran in a tropical hypereutrophic lake. Aquat Ecol 53(3): 453–464. doi.org/10.1007/s10452-019-09701-8.

CHISLOCK, M.F., KAUL, K.A., DURHAM, K.A., SARNELLE, O., WILSON, A.E. 2019. Eutrophication mediates rapid clonal evolution in Daphnia pulicaria. Freshw. Biol. 64: 1275 – 1283. doi.org/10.1111/fwb.13303

FERRÃO-FILHO, A.S; COSTA, S. M.; RIBEIRO, A.D. (2009). Biomonitoring of cyanotoxins in two tropical reservois by cladocera toxicity bioassays. Ecotoxicology and Envionmental Safety.

FERRÃO-FILHO, A.S; KOZLOWSKY-SUZUKI, B (2011). Cyanotoxins: Bioaccumulation and effects on aquatic animals. Mar Drugs 9: 2729–2772. doi.org/10.3390/md9122729

FERRÃO-FILHO, A. DA S., & DA SILVA, D. A. C. (2020). Saxitoxin-producing Raphidiopsis raciborskii (cyanobacteria) inhibits swimming and physiological parameters in Daphnia similis. Science of The Total Environment, 135751. doi:10.1016/j.scitotenv.2020.1357

FERRÃO-FILHO, A.S; DIAS, T.M; PEREIRA, U.J; SANTOS, J.A; KOZLOWSKY-SUZUKI, B. (2019) Nutritional and toxicity constraints of phytoplankton from a Brazilian reservoir to the fitness of cladoceran species. Environ Sci Pollut R 26(13): 12881–12893. doi.org/10.1007/s11356-019-04851-6.

GER, K.A; URRUTIA-CORDERO P; FROST, P.C; HANSSON, L.A; SARNELLE O, WILSON, A.E; LÜRLING M (2016) The interaction between cyanobacteria and zooplankton in a more eutrophic world. Harmful Algae 54: 128–144. doi.org/10.1016/j.hal.2015.12.005.

HOLLAND, A.; KINNEAR, S. (2013) Interpreting the Possible Ecological Role (s) of Cyanotoxins: Compounds for Competitive Advantage and/or Physiological Aide? Mar Drugs 11: 2239–2258.

KIM, M.S., LEE, C., 2019. Ozonation of microcystins: Kinetics and toxicity decrease. Environ. Sci. Technol. 53, 6427-6435. https://doi.org/10.1021/acs.est.8b06645

KOSIBA, J., KRZTOŃ, W., & WILK-WOŹNIAK, E., 2018. Effect of Microcystins on Proto- and Metazooplankton Is More Evident in Artificial Than in Natural Waterbodies. Microbial Ecology. 75(2), 293–302. doi:10.1007/s00248-017-1058-z.


quality determines lipase gene expression and lipase/esterase activity in Daphnia pulex. Biology open, 6(2), 210-216

LYU, K., GU, L., BANGPING, L., YICHUN, L., CHANGCAN, W., GUAN, H., YANG, Z. 2019. Stress-responsive expression of a glutathione S-tranferase (delta) gene in waterflea Daphnia magna challenged by microcystin-producing and microcytin-free Microcystis aeruginosa. Harmful Algae, 56: 1 – 8.

OMIDI, A., ESTERHUIZEN-LONDT, M. PFLUGMACHER, S. 2018. Still challenging: the ecological function of the cyanobacterial toxin microcystin – What we know so far. Toxin Reviews, 37(2): 87-105

ORTIZ-RODRÍGUEZ, R., & WIEGAND, C. (2010). Age related acute effects of microcystin-LR on Daphnia magna biotransformation and oxidative stress. Toxicon, 56(8), 1342–1349. doi:10.1016/j.toxicon.2010.07.020

PAWLIK-SKOWROŃSKA, B., TOPOROWSKA, M., & MAZUR-MARZEC, H. (2019). Effects of secondary metabolites produced by different cyanobacterial populations on the freshwater zooplankters Brachionus calyciflorus and Daphnia pulex. Environmental Science and Pollution Research. doi:10.1007/s11356-019-04543.

POWER, E.A.; BOUMPHREY, R.S. Ecotoxicology, International Trends in Bioassay Use for Effluent Management, 13, 377–398, 2004.

RANGEL, L. M., SILVA, L. H., FAASSEN, E. J., LÜRLING, M., GER, K. A. (2020).

Copepod Prey Selection and Grazing Efficiency Mediated by Chemical and Morphological Defensive Traits of Cyanobacteria. Toxins, 12(7), 465. doi:10.3390/toxins12070465

RIZZO, L. Bioassays as a tool for evaluating advanced oxidation process in water and wastewater treatment. Water Research, v. 45, p. 4311-4340, 2011.

SAVIC, B. G., BORMANS, M., EDWARDS, C., LAWTON, L., BRIAND, E., & WIEGAND, C. Cross talk: Two way allelopathic interactions between toxic Microcystis and Daphnia. Harmful Algae, 94, 2020. 101803. doi:10.1016/j.hal.2020.101803.

SAVIC, G.B; COLINET, H; BORMANS, M; EDWARDS, C; LINDA, A; LAWTON, B.; WIEGAND, C. Cell free Microcystis aeruginosa spent medium affects Daphnia magna survival and stress response. Toxicon. 195 (2021) 37–47. 10.1016/j.toxicon.2021.03.009.

SHAHMOHAMADLOO, R. S., POIRIER, D. G., ORTIZ ALMIRALL, X., BHAVSAR, S. P., & SIBLEY, P. K. (2019). Assessing the toxicity of cell-bound microcystins on freshwater pelagic and benthic invertebrates. Ecotoxicology and Environmental Safety, 109945. doi:10.1016/j.ecoenv.2019.109945

WIECZERZAK, M.; NAMIESNIK, J.; KUDLAK, B. Bioassays as one of the Green Chemistry tools for assessing environmental quality: A review. Environment International, v. 94, p. 341-361, 2016

ZAGATTO, P.A.; BURATINI S.A.; ARAGÃO M., FERRÃOFILHO; A.S. 2012. Neurotoxicity of two Cylindrospermopsis raciborskii strains to mice, Daphnia and fish. Environ Toxicol Chem 31: 857 – 862. https://doi.org/10.1002/etc.1759

ZANCHETT G, OLIVEIRA-FILHO EC, 2013. Cyanobacteria and cyanotoxins: from impacts on aquatic ecosystems and human health to anticarcinogenic effects. Toxins (Basel) 5 (10), 1896–1917.




How to Cite

Albuquerque, M. V. da C., Dias, J., Batista, F. R. da C., Oliveira, E. M. A., e Silva, M. C. C. de P., Rodrigues, R. M. M., Leite, V. D., & Lopes, W. S. (2023). Daphnias spp. as bioindicator organisms of toxicity and environmental characterization of eutrophized aquatic systems. OBSERVATÓRIO DE LA ECONOMÍA LATINOAMERICANA, 21(10), 17419–17431. https://doi.org/10.55905/oelv21n10-156




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