Bioactivity of essential oils in the control of Sitophilus ssp.


  • Guilherme Hammarstrom Dobler
  • Gabriella Medianeira Machado de Oliveira
  • Neusa Fernandes de Moura



bioactive compounds, terpenes, insect control, chromatography


Brazil is the largest rice producer outside of Asia, and the large production of this grain in the Brazilian territory has aspects that affect its productivity. Among all the influencing aspects is the rice weevil, Sitophilus ssp. Considering the needs of the grain industry and the market that seeks products without toxic residues, the work evaluated the bioactivity of the essential oils of laurel (Laurus nobilis), eucalyptus (Eucalyptus citriodora), and mint (Mentha piperita) on the coleopteran Sitophilus ssp. The insects used were obtained from infested silos and kept in a breeding room at a controlled temperature. For testing the bioactivity of essential oils, essential oils were used at concentrations of 1%, 5%, and 10%. The adult insects used were placed on the plates and the count of live individuals (rice weevil) was performed at times of 15 min., 30 min., 60 min., 6h, 12h, and 24h. All tested oils showed efficiency in insect mortality. The chemical characterization of essential oils was performed by gas chromatography coupled with mass spectrometry, indicating the presence of oxygenated monoterpenes in the three essential oils analyzed.


AGGARWAL, K. K. et al. Toxicity of 1,8-cineole towards three species of stored product coleopterans. International Journal of Tropical Insect Science, v. 21, n. 02, p. 155–160, 19 jun. 2001a.

AGGARWAL, K. K. et al. Toxicity of 1,8-cineole towards three species of stored product coleopterans. International Journal of Tropical Insect Science, v. 21, n. 02, p. 155–160, 19 jun. 2001b.

AISSAOUI, L. et al. Phytochemical screening and biological effects of laurus nobilis (lauraceae) essential oil against mosquito larvae, Culex Pipiens (linneaus, 1758) (diptera: culicidae) species. Applied Ecology and Environmental Research, v. 21, n. 1, p. 287–300, 2023.

AK SAKALLI, E. et al. In vitro and in silico Evaluation of ACE2 and LOX Inhibitory Activity of Eucalyptus Essential Oils, 1,8-Cineole, and Citronellal. Natural Product Communications, v. 17, n. 6, p. 1934578X2211094, 23 jun. 2022.

AMRI, I. et al. Essential Oils and Biological Activities of Eucalyptus falcata, E. sideroxylon and E. citriodora Growing in Tunisia. Plants, v. 12, n. 4, p. 816, 11 fev. 2023.

BRARI, J.; KUMAR, V. Insecticidal Potential of Two Monoterpenes against Tribolium Castaneum (Herbst.) and Sitophilus Oryzae (L.) Major Stored Product Insect PestsInternational Journal of Pharmaceutical & Biological Archives. [s.l: s.n.]. Disponível em: <>.

BRONDANI, G.; VEY, I. H.; CARDONA VENTURINI, J. Diferenciais e custos em culturas de arroz: a experiência do Rio Grande do Sul. n. 1, p. 61–74, 2006.

CAMELE, I.; GRUĽOVÁ, D.; ELSHAFIE, H. S. Chemical Composition and Antimicrobial Properties of Mentha × piperita cv. ‘Kristinka’ Essential Oil. Plants, v. 10, n. 8, p. 1567, 30 jul. 2021.

CAPATTI, C. E.; MACRON, R. K.; MACHADO, M. B. Avaliação de dano de Sitophilus Oryzaephilus surinamensis e Laemophloeus minutusem grãos de arroz armazenados. Revista Brasileira de Engenharia Agrícola e Ambiental, v. 17, n. 8, p. 855–860, 2013.

CAPUTO, L. et al. Laurus nobilis: Composition of Essential Oil and Its Biological Activities. Molecules, v. 22, n. 6, p. 930, 3 jun. 2017.

DE ALBUQUERQUE LIMA, T. et al. Insecticidal activity of a chemotype VI essential oil from Lippia alba leaves collected at Caatinga and the major compound (1,8-cineole) against Nasutitermes corniger and Sitophilus zeamais. Pesticide Biochemistry and Physiology, v. 177, p. 104901, ago. 2021.

DEVI, M. A. et al. Chemical compositions and insecticidal efficacies of four aromatic essential oils on rice weevil Sitophilus oryzae L. International Journal of Tropical Insect Science, v. 40, n. 3, p. 549–559, 10 set. 2020.

FAO. World Food and Agriculture – Statistical Yearbook 2021. [s.l.] FAO, 2021.

FIDAN, H. et al. Chemical Composition and Antimicrobial Activityof Laurus nobilis L. Essential Oils from Bulgaria. Molecules, v. 24, n. 4, p. 804, 22 fev. 2019.

FRANZ, A. R.; KNAAK, N.; FIUZA, L. M. Toxic effects of essential plant oils in adult Sitophilus oryzae (Linnaeus) (Coleoptera, Curculionidae). Revista Brasileira de Entomologia, v. 55, n. 1, p. 116–120, mar. 2011.

HAMAD AL-MIJALLI, S. et al. Determination of Volatile Compounds of Mentha piperita and Lavandula multifida and Investigation of Their Antibacterial, Antioxidant, and Antidiabetic Properties. Evidence-Based Complementary and Alternative Medicine, v. 2022, p. 1–9, 14 jun. 2022.

KARAKOSTA, L. K. et al. Combined Effect of Chitosan Coating and Laurel Essential Oil (Laurus nobilis) on the Microbiological, Chemical, and Sensory Attributes of Water Buffalo Meat. Foods, v. 11, n. 11, p. 1664, 6 jun. 2022.

LIU, Z. L.; CHU, S. S.; JIANG, G. H. Toxicity of Schizonpeta multifida essential oil and its constituent compounds towards two grain storage insects. Journal of the Science of Food and Agriculture, v. 91, n. 5, p. 905–909, 30 mar. 2011.

LÓPEZ, M. D.; PASCUAL-VILLALOBOS, M. J. Mode of inhibition of acetylcholinesterase by monoterpenoids and implications for pest control. Industrial Crops and Products, v. 31, n. 2, p. 284–288, mar. 2010.

LOUMOUAMOU, A. N. et al. Yield and Composition of Essential Oils From Eucalyptus citriodora x Eucalyptus torelliana, a Hybrid Species Growing in Congo-Brazzaville. Journal of Essential Oil Research, v. 21, n. 4, p. 295–299, jul. 2009.

MACCHIONI, F. et al. Composition and Acaricidal Activity of Laurus novocanariensis and Laurus nobilis Essential Oils Against Psoroptes cuniculi. Journal of Essential Oil Research, v. 18, n. 1, p. 111–114, jan. 2006.

MARCHEZAN, E.; MENEZES, N. L. DE; SIQUEIRA, C. DO A. Controle da qualidade das sementes de arroz irrigado utilizadas em Santa Maria/RS. n. 3, p. 375–379, 2001.

MILANI, E. DE A. C. Revisão: ozonização no controle da incidência de pragas em grãos armazenados. Monografia—Brasília: Universidade de Brasília, 2021.

MOGHADDAM, M. et al. Composition and Antifungal Activity of Peppermint (Mentha piperita) Essential Oil from Iran. Journal of Essential Oil Bearing Plants, v. 16, n. 4, p. 506–512, 4 jul. 2013.

OOTANI, M. A. et al. TOXICIDADE DE ÓLEOS ESSENCIAIS DE EUCALIPTO E CITRONELA SOBRE Sitophilus zeamais MOTSCHULSKY (Coleoptera: Curculionidae). Original Article Biosci. J, v. 27, n. 4, p. 609–618, 2011.

ÖZOGUL, Y.; EL ABED, N.; ÖZOGUL, F. Antimicrobial effect of laurel essential oil nanoemulsion on food-borne pathogens and fish spoilage bacteria. Food Chemistry, v. 368, p. 130831, jan. 2022.

PANG, X. et al. Toxicity and repellent activity of essential oil from Mentha piperita Linn. leaves and its major monoterpenoids against three stored product insects. Environmental Science and Pollution Research, v. 27, n. 7, p. 7618–7627, 29 mar. 2020.

PATIÑO-BAYONA, W. R. et al. Effects of Essential Oils from 24 Plant Species on Sitophilus zeamais Motsch (Coleoptera, Curculionidae). Insects, v. 12, n. 6, p. 532, 8 jun. 2021.

RENOZ, F. et al. The modes of action of Mentha arvensis essential oil on the granary weevil Sitophilus granarius revealed by a label-free quantitative proteomic analysis. Journal of Pest Science, v. 95, n. 1, p. 381–395, 28 jan. 2022.

RODRIGUES, G. A. C. Preferência Alimentar de Sitophilus oryzae (Linnaeus, 1763) (Coleoptera: Dryophthoridae) a diferentes variedades de arroz e avaliação de perdas. Monografia—Rio Claro: Universidade Estadual Paulista Júlio de Mesquita Filho, 2013.

SAHIN BASAK, S.; CANDAN, F. Effect of Laurus nobilis L. Essential Oil and its Main Components on α-glucosidase and Reactive Oxygen Species Scavenging Activity. Iranian journal of pharmaceutical research : IJPR, v. 12, n. 2, p. 367–79, 2013.

SCHMOELER, M. Presença de artrópodes em alimentos estocados: uma revisão bibliográfica. Florianópolis: [s.n.].

SHOKOOHINIA, Y. et al. Seasonal variations of Laurus nobilis L. leaves volatile oil components in Isfahan, IranResearch Journal of Pharmacognosy (RJP). [s.l: s.n.]. Disponível em: <>.

SILVA, A. O. DA et al. Armazenamento de grãos na agricultura familiar: principais problemáticas e formas de armazenamento na região nordeste paraense. Research, Society and Development, v. 10, n. 1, p. e36610111835, 17 jan. 2021.

TADA, J. M.; NARDI, C. P. P. DE. Caruncho (Sitophilus sp.) como veiculador de micro-organismos patogênicos no arroz e no milho. Matão: [s.n.].

WATTO, M. A.; MUGERA, A. W. Measuring Production and Irrigation Efficiencies of Rice Farms: Evidence from the Punjab Province, Pakistan. Asian Economic Journal, v. 28, n. 3, p. 301–322, 2014.




How to Cite

Dobler, G. H., de Oliveira, G. M. M., & de Moura, N. F. (2023). Bioactivity of essential oils in the control of Sitophilus ssp. OBSERVATÓRIO DE LA ECONOMÍA LATINOAMERICANA, 21(11), 20897–20909.