Avaliação de sistema aquoso bifásico para extração de β glucanas de Lentinula edodes
DOI:
https://doi.org/10.55905/oelv21n11-062Keywords:
cogumelo shiitake, sistemas bifásicos, β glucanosAbstract
Os fungos medicinais, particularmente o Shiitake (L. edodes), têm sido altamente considerados nas tradições orientais devido aos seus efeitos vantajosos no tratamento de diversos problemas de saúde. O objetivo geral desse projeto foi avaliar a extração de β glucanas do L. Edodes por sistema bifásico. Os cogumelos frescos foram cortados em cubos e processados com um moinho de facas e martelos, e depois moídos finamente em seguida o material resultante foi colocado em um percolador com etanol 70% e concentrado em rotaevaporador. Posteriormente, foi ainda concentrado e liofilizado. Várias concentrações de PEG 600 (50%) e Na2SO4 (20%) foram utilizadas para criar sistemas, visando investigar configurações bifásicas e não bifásicas. Optou-se por sistemas bifásicos para extração de β-glucanas e remoção de proteínas de L. edodes (ambos liofilizados e em sua solução 20 mg/mL). As fases separadas foram coletadas e analisadas quanto aos níveis de proteína total e β-glucana. Os resultados demonstraram, que foi possível a remoção de proteínas totais e extração de β glucanas. Nossos resultados indicam que o sistema B foi o mais eficaz na remoção de proteínas e extração bem-sucedida das β-glucanas por meio da técnica de extração líquido-líquido. O uso de um sistema aquoso bifásico mostrou-se uma alternativa promissora para a extração de compostos ativos de cogumelos.
References
ANDRADE, M. C. N. DE; MINHONI, M. T. DE A.; ZIED, D. C. Caracterização bromatológica de oito linhagens de Lentinula edodes (Shiitake) cultivadas em toras de Eucalyptus grandis. Food Science and Technology, v. 28, p. 793–797, 2008.
BAUMANN, P.; BAUMGARTNER, K.; HUBBUCH, J. Influence of binding pH and protein solubility on the dynamic binding capacity in hydrophobic interaction chromatography. Journal of Chromatography, v. 1396, p. 77–85, 2015.
CHANG, S; BUSWELL, J. “Medicinal Mushrooms: Past, Present and Future.” Advances in biochemical engineering/biotechnology, v. 184, p. 1-27, 2023.
CHENG, P. G. et al. Polysaccharides-Rich Extract ofGanoderma lucidum(M.A. Curtis:Fr.) P. Karst Accelerates Wound Healing in Streptozotocin-Induced Diabetic Rats. Evidence-Based Complementary and Alternative Medicine, v. 2013, p. 1–9, 2013.
DE JESUS, L. I. et al. Simple and effective purification approach to dissociate mixed water-insoluble α- and β-D-glucans and its application on the medicinal mushroom Fomitopsis betulina. Carbohydrate Polymers, v. 200, p. 353–360, 2018.
DENG, P. et al. Worldwide Research Trends on Diabetic Foot Ulcers (2004-2020): Suggestions for Researchers. J Diabetes Res, v. 7, n. 2022, p.7991031, 2022.
SILVA, D. D. D. et al. Medicinal mushrooms in prevention and control of diabetes mellitus. Fungal Diversity, v. 56, n. 1, p. 1-29, 2012.
DI NASO, F. C. et al. Effect of Agaricus blazei Murill on the pulmonary tissue of animals with streptozotocin-induced diabetes. Experimental Diabetes Research, v. 2010, p. 1-8, 2010.
FIEDLER, U.; AUGUSTIN, H. G. Angiopoietins: a link between angiogenesis and inflammation. Trends in Immunology, v. 27, n. 12, p. 552–558, 2006.
WANG, H.-J. et al. Hypoglycemic effect of crude exopolysaccharides produced by a medicinal mushroom Phellinus baumii in streptozotocin-induced diabetic rats. Life Sciences, v. 76, n. 26, p. 3069–3080, 2005.
KIM, D.-H. et al. A Preliminary Study on the Hypoglycemic Effect of the Exo - Polymers Produced by Five Different Medicinal Mushrooms. Journal of Microbiology and Biotechnology, v. 11, n. 1, p. 167–171, 2001.
KWON, A.-H. et al. Effects of medicinal mushroom (Sparassis crispa) on wound healing in streptozotocin-induced diabetic rats. The American Journal of Surgery, v. 197, n. 4, p. 503–509, 2009.
LEE, J. S.; HONG, E. K. Immunostimulating activity of the polysaccharides isolated from Cordyceps militaris. International Immunopharmacology, v. 11, n. 9, p. 1226–1233, 2011.
LI, J. et al. Alkaline Extraction, Structural Characterization, and Bioactivities of (1→6)-β-d-Glucan from Lentinus edodes. Molecules, v. 24, n. 8, p. 1610, 24 abr. 2019.
LO, H.-C.; WASSER, S. P. Medicinal Mushrooms for Glycemic Control in Diabetes Mellitus: History, Current Status, Future Perspectives, and Unsolved Problems (Review). International Journal of Medicinal Mushrooms, v. 13, n. 5, p. 401–426, 2011.
MIHAILOVIĆ, M. et al. Corrigendum to “Protective Effects of the Mushroom Lactarius deterrimus Extract on Systemic Oxidative Stress and Pancreatic Islets in
Streptozotocin-Induced Diabetic Rats”. Journal of Diabetes Research, v. 2017, p. 1–2, 2017.
MORALES, D. et al. Isolation and comparison of α- and β-D-glucans from shiitake mushrooms (Lentinula edodes) with different biological activities. Carbohydrate Polymers, v. 229, p. 115521, 2020.
MORALES, D. Testing the effect of combining innovative extraction technologies on the biological activities of obtained β-glucan-enriched fractions from Lentinula edodes. Journal of Functional Foods, v. 60, p. 103446, 2019.
RODRIGUES, H. G. et al. Oral Administration of Linoleic Acid Induces New Vessel Formation and Improves Skin Wound Healing in Diabetic Rats. v. 11, n. 10, p. e0165115, 2016.
TIAN, M. et al. The Relationship Between Inflammation and Impaired Wound Healing in a Diabetic Rat Burn Model. Journal of Burn Care & Research: Official Publication of the American Burn Association, v. 37, n. 2, p. e115-124, 2016.
WANG, J. et al. Structural Characterization and Chain Conformation of Water-Soluble β-Glucan from Wild Cordyceps sinensis. Journal of Agricultural and Food Chemistry, v. 67, n. 45, p. 12520–12527, 2019.
WHISTLER, R. L. Solubility of Polysaccharides and Their Behavior in Solution. Advances in Chemistry, p. 242–255, 1973
YAMAMOTO, K.; KIMURA, T. Orally and topically administered Sparassis crispa (Hanabiratake) improved healing of skin wounds in mice with streptozotocin-induced diabetes. Bioscience, Biotechnology, and Biochemistry, v. 77, n. 6, p. 1303–1305, 2013.
ZHONG, J.-J.; XIAO, J.-H. Secondary Metabolites from Higher Fungi: Discovery, Bioactivity, and Bioproduction. Biotechnology in China I, v. 123, p. 79–150, 2009.
ZYKOVA, S. N. et al. Macrophage stimulating agent soluble yeast β‐1,3/1,6‐glucan as a topical treatment of diabetic foot and leg ulcers: A randomized, double blind, placebo‐controlled phase II study. Journal of Diabetes Investigation, v. 5, n. 4, p. 392–399, 2013.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 OBSERVATÓRIO DE LA ECONOMÍA LATINOAMERICANA
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
