Bioactive compounds contents of and antioxidant activity of Nectan-dra megapotamica (Lauraceae)
DOI:
https://doi.org/10.55905/oelv21n8-132Keywords:
phytochemistry, free radicals, phenolsAbstract
Nectandra megapotamica (Spreng.) Mez., Lauraceae known as cinnamon, is a species native to Brazil, widely used in folk medicine. The present project aimed to evaluate the antioxidant activity and determine the contents of phenols, flavonoids, tannins, and total alkaloids in extracts from the leaves and bark of a specimen of N. megapotamica that occurs in the southern region of the state of Mato Grosso do Sul. Crude ethanolic extracts obtained from leaves and bark were tested for antioxidant activity by DPPH and ABTS methods and dosage of phenols, flavonoids, tannins, and total alkaloids by spectrophotometry in the visible region. The extracts exhibited considerable antioxidant activity, particularly the leaf extract was the most active in both methods, yielding IC50 of 9.0 ± 0.18 and 14.29 ± 0.16 μg.mL-1, respectively. This extract showed higher levels of phenols, flavonoids, tannins, and total alkaloids. The results suggest that the antioxidant activity is related to the presence of phenolic compounds and alkaloids, known antioxidant compounds, quantified in this study.
References
ALMEIDA, K. C. R. et al. Biological properties and chemical composition of essential oil from Nectandra megapotamica (Spreng.) Mez. leaves (Lauraceae). Nat Prod Res., v. 34, n. 21, p. 3149-3153, 2020. https://doi.org/10.1080/14786419.2019.1608539.
ALVES, O. E. et al. Levantamento etnobotânico e caracterização de plantas medicinais em fragmentos florestais de Dourados-MS. Ciênc. agrotec., v. 32, n. 2, p. 651-658, 2008. https://doi.org/10.1590/S1413-70542008000200048.
ARNOSO, B. J. M.; COSTA, G. F.; SCHMIDT, B. Biodisponibilidade e classificação de compostos fenólicos. Nutrição Brasil, v. 18, n. 1, p. 39-48, 2019. https://doi.org/10.33233/nb.v18i1.1432.
BARBOSA, K. B. F. et al. Estresse oxidativo: conceito, implicações e fatores modulatórios. Rev. de Nutr., v. 23, n. 4, p. 629-643, 2010. https://doi.org/10.1590/S1415-52732010000400013.
CAI, Y. et al. Recent advances in anticancer activities and drug delivery systems of tannins. Med Res Rev., v. 37, n. 4, p. 665-701, 2017. https://doi.org/10.1002/med.21422.
CHEN, Y. F. et al. Alkaloids and flavonoids exert protective effects against UVB-induced damage in a 3D skin model using human keratinocytes. Results in Chem., v. 4, 2022. https://doi.org/10.1016/j.rechem.2022.100298.
DANIELLI, L. J. et al. Influence of Monoterpenes in biological activities of Nectandra megapotamica (Spreng.) Mez essential oils. Biomolecules, v. 9, n.3, p. 112, 2019. https://doi.org/10.3390/biom9030112.
DA SILVA FILHO, A. A. et al. Tetrahydrofuran lignans from Nectandra megapotamica with trypanocidal activity. J. Nat. Prod. v. 67, n. 1, p. 42-45, 2004. https://doi.org/10.1021/np0302697.
El, S. N.; KARAKAYA, S. Radical scavenging and iron-chelating activities of some greens used as traditional dishes in Mediterranean diet. Int J Food Sci Nutr., v. 55, n. 1, p. 67-74, 2004. https://doi.org/10.1080/09637480310001642501.
FERREIRA, L. A. O. et al. Antileishmanial and antioxidant potential of fractions and isolated compounds from Nectandra cuspidata. Nat. Prod. Res., v. 32, n. 23, p. 2825-2828, 2018. https://doi.org/10.1080/14786419.2017.1378214.
GAN, J. et al. Correlations between antioxidant activity and alkaloids and phenols of Maca (Lepidium meyenii). J. Food Qual., v. 2017, n. 3, p. 1-10. https://doi.org/10.1155/2017/3185945.
GARCEZ, F. R. et al. Fenilpropanóides e outros constituintes bioativos de Nectandra megapotamica. Quim. Nova, v. 32, n. 2, p. 407-411, 2009. https://doi.org/10.1590/S0100-40422009000200026.
GONZÁLEZ, L. M.; MOSQUERA, O. M. Nectandra acutifolia (Ruiz & Pav.) Mez (Lauraceae) Reduces oxidative stress induced with Rotenone in Drosophila melanogaster. J. Pharm. Pharmacol., v. 7, n. 12, p. 599-610, 2019. https://doi.org/10.17265/2328-2150/2019.12.001.
GÜLÇIN, I. et al. Antioxidant activity of bisbenzylisoquinoline alkaloids from Stephania rotunda: cepharanthine and fangchinoline. J Enzyme Inhib Med Chem, v. 25, n. 1, p. 44-53, 2010a. https://doi.org/10.3109/14756360902932792.
GÜLÇIN, H. et al. Radical scavenging and antioxidant activity of tannic acid. Arab. J. Chem., v. 3, n. 1, p. 43-53, 2010b. https://doi.org/10.1016/j.arabjc.2009.12.008.
HAIDA, S.; KRIBII, A.; KRIBII, A. Chemical composition, phenolic content and antioxidant capacity of Haloxylon scoparium extracts. S. Afr. J. Bot., v. 131, p. 151-160, 2020. https://doi.org/10.1016/j.sajb.2020.01.037.
HEINRICH, M.; MAH, J.; AMIRKIA, V. Alkaloids used as medicines: structural phytochemistry meets biodiversity-An update and forward look. Molecules, v. 26, n. 7, 2021. https://doi.org/10.3390/molecules26071836.
LIN, J. Y.; TANG, C. Y. Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food Chem., v. 101, n. 1, p. 140-147, 2007. https://doi.org/10.1016/j.foodchem.2006.01.014.
LORENZI, H. Árvores brasileiras: Manual de identificação e cultivo de plantas arbóreas nativas do Brasil. Nova Odessa, SP, Plantarum, 368p. 1998.
MACÁKOVÁ, K. et al. The influence of alkaloids on oxidative stress and related signaling pathways. Free Radic. Biol. Med., v. 134, p. 429-444, 2019. https://doi.org/10.1016/j.freeradbiomed.2019.01.026.
MONEIM, E. A. et al. Quantitative determination of tannin content in some Sorghum cultivars and evaluation of its antimicrobial activity. Res. J. Microbiol., v. 2, n. 3, p. 284-288, 2007. https://scialert.net/abstract/?doi=jm.2007.284.288.
MORAES, G. V. et al. Potencial antioxidante dos flavonoides e aplicações terapêuticas. Res., Soc. Dev., v. 11, n. 14, 2022. http://dx.doi.org/10.33448/rsd-v11i14.36225.
NOREEN, H. et al. Measurement of total phenolic content and antioxidant activity of aerial parts of medicinal plant Coronopus didymus. Asian Pac. J. Trop. Med., v, 10, n. 8, p. 723-834, 2017. https://doi.org/10.1016/j.apjtm.2017.07.024.
PAZINATTO, B. C.; MARQUES, D. T.; LIMA, C. P. Avaliação da atividade antioxidante e compostos bioativos presentes em extratos de beterraba. Cad. da Esc. de Saúde, v. 21, n.1, p. 44-56 44. 2022. https://doi.org/10.25192/issn.1984-7041.v21i16356.
PEINADO, N. M. et al. Anti-Trypanosoma cruzi activity of alkaloids isolated from Habranthus brachyandrus (Amaryllidaceae) from Argentina. Phytomedicine, v. 101, 2022. https://doi.org/10.1016/j.phymed.2022.154126.
PIETTA, P. G. Flavonoides como antioxidantes. J. Nat. Prod., v. 63, n. 7, p. 1035-1042, 2000. http://dx.doi.org/10.1021/np9904509.
PONCI, V. et al. Neolignans from Nectandra megapotamica (Lauraceae) Display in vitro cytotoxic activity and induce apoptosis in leukemia cells. Molecules, v. 20, n. 7, p. 12757-12768, 2015. https://doi.org/10.3390/molecules200712757.
SALACHNA, P.; PIETRAK, A.; LOPUSIEWICZ, L. Antioxidant potential of flower extracts from Centaurea spp. depends on their content of phenolics, flavonoids and free amino acids. Molecules, v. 26, n. 24, 2021. https://doi.org/10.3390/molecules26247465.
SILVA FILHO, A. A. In vitro antileishmanial and antimalarial activities of tetrahydrofuran lignans isolated from Nectandra megapotamica (Lauraceae). Phytother Res., v. 22, n. 10, p. 1307- 1310, 2008. https://doi.org/10.1002/ptr.2486.
SILVA FILHO, A. A. et al. Evaluation of analgesic and anti-inflammatory activities of Nectandra megapotamica (Lauraceae) in mice and rats. J Pharm Pharmacol., v. 56, n. 9, p. 1179-1184, 2004. https://doi.org/10.1211/0022357044058.
SILVA, D. T. et al. Nectandra grandiflora by-products obtained by alternative extraction methods as a source of phytochemicals with antioxidant and antifungal properties. Molecules. v. 23, n. 2, p. 372, 2018. https://doi.org/10.3390/molecules23020372.
SINGH, D. K.; SRIVASTAVA B.; SAHU A. Spectrophotometric determination of Rauwolfia alkaloids: estimation of reserpine in pharmaceuticals. Anal. Sci., v. 20, p. 571-573, 2004. https://doi.org/10.2116/analsci.20.571.
SOUSA, C. M. M. et al. Fenóis totais e atividade antioxidante de cinco plantas medicinais. Quim. Nova, v. 30, n. 2, p. 351-355, 2007. https://doi.org/10.1590/S0100-40422007000200021.
TELES, A. M. et al. Fenólicos totais e atividade antioxidante do extrato obtido do fungo Penicillium purpurogenum advindo de ambiente marinho poluído do Maranhão. Res., Soc. Dev., v. 9, n. 8, 2020. https://doi.org/10.33448/rsd-v9i8.6205.
VASCONCELOS, N. G. et al. Antibacterial activity and synergism of the essential oil of Nectandra megapotamica (L.) flowers against OXA-23-producing Acinetobacter baumannii. J. Essent. Oil Res., v. 32, n. 3, p. 1-9, 2020. https://doi.org/10.1080/10412905.2020.1740802.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
